Kido A, Kimura Y, Nishizono T, Inoue T, Oya M
Department of Legal Medicine, Yamanashi Medical University, Japan.
Nihon Hoigaku Zasshi. 1994 Aug;48(4):263-6.
By means of non-immobilized isoelectric focusing followed by immunoblotting, group-specific component (GC) subtypes were detected in dental pulp tissues. The amount of GC in dental pulp was quantitated using rocket immunoelectrophoresis and the minimum amount of the protein necessary for the typing of GC was estimated to be about 0.02 microgram in 10 microliters of the sample. The results of GC typing in dental pulp tissues were in agreement with the phenotypes obtained from serum samples of the same individuals. Subtyping by the present method was also possible in dental pulp tissues obtained from the teeth stored for 4 weeks at room temperature. The GC subtyping by non-immobilized isoelectric focusing is of practical use for medicolegal investigation of teeth.
通过非固定化等电聚焦随后进行免疫印迹法，在牙髓组织中检测到了组特异性成分（GC）亚型。使用火箭免疫电泳对牙髓中的GC量进行定量，估计在10微升样品中进行GC分型所需的蛋白质最小量约为0.02微克。牙髓组织中GC分型的结果与同一受试者血清样本所获得的表型一致。用本法进行亚型分析在室温下保存4周的牙齿所获取的牙髓组织中也是可行的。通过非固定化等电聚焦进行GC亚型分析在牙齿法医学调查中具有实际应用价值。
