Kido A, Kimura Y, Oya M
Department of Legal Medicine, Yamanashi Medical University, Japan.
Electrophoresis. 1995 Jun;16(6):1024-6. doi: 10.1002/elps.11501601172.
Group-specific component (GC) subtyping in semen and seminal stains was carried out using isoelectric focusing in carrier ampholyte-generated pH gradients and immunoblotting. In serum samples the anodal bands of GC 1F and of GC 1S disappeared by neuraminidase treatment, but in semen samples these bands remained unchanged after such treatment. The GC 2 type in semen exhibited two bands: the main GC 2 band and another fast band which focused at the position of the cathodic band of GC 1F. These seminal GC bands were unaffected by enzyme digestion. Reliable subtyping was possible in seminal stains stored at 4 degrees C for up to 10 weeks, at room temperature for up to 8 weeks, and at 37 degrees C for up to 5 weeks. The GC subtyping by conventional isoelectric focusing after neuraminidase treatment is simple, economical and useful in medicolegal examination of seminal stains.
采用载体两性电解质形成pH梯度的等电聚焦和免疫印迹法对精液和精斑进行群体特异性成分（GC）分型。在血清样本中，GC 1F和GC 1S的阳极带经神经氨酸酶处理后消失，但在精液样本中，此类处理后这些带保持不变。精液中的GC 2型呈现两条带：主要的GC 2带和另一条快速带，其聚焦于GC 1F阴极带的位置。这些精液GC带不受酶消化的影响。对于保存在4℃长达10周、室温下长达8周以及37℃长达5周的精斑，均可进行可靠的分型。经神经氨酸酶处理后，通过传统等电聚焦进行GC分型在精斑的法医检验中简单、经济且实用。
