波动试验作为一种更灵敏的系统，用于测定L5178Y小鼠淋巴瘤细胞中的诱导突变。

The fluctuation test as a more sensitive system for determining induced mutation in L5178Y mouse lymphoma cells.

作者信息

Cole J, Arlett C F, Green M H

出版信息

Mutat Res. 1976 Dec;41(2-3):377-86. doi: 10.1016/0027-5107(76)90110-x.

Abstract

Luria-Delbrück fluctuation tests for the determination of the spontaneous mutation rate to ouabain resistance incultured L5178Y mouse lymphoma cells gave values in the range 0.44 to 1.03 X 10(-7) mutants per cell per generation. Addition of very low, non-toxic, levels of EMS (0.1 mM) and MMS (0.012 mM) 30-48 h before plating in selective medium gave a highly significant increase in the number of ouabain-resistant mutants. Methods for the calculation of spontaneous and induced mutation rates are discussed and a method for the computation of induced mutation rates is described. It is suggested that the modified fluctuation test is a simpler and considerably more sensitive assay for mutagens than the conventional experimental design. Some of its limitations are discussed.

摘要

用于测定培养的L5178Y小鼠淋巴瘤细胞对哇巴因抗性的自发突变率的鲁里亚-德尔布吕克波动试验得出的值为每细胞每代0.44至1.03×10⁻⁷个突变体。在接种于选择培养基前30 - 48小时添加极低、无毒水平的甲基磺酸乙酯（EMS，0.1 mM）和甲基磺酸甲酯（MMS，0.012 mM），使哇巴因抗性突变体的数量显著增加。讨论了自发和诱导突变率的计算方法，并描述了一种计算诱导突变率的方法。有人提出，改良的波动试验对于诱变剂来说是一种比传统实验设计更简单且灵敏度高得多的检测方法。还讨论了其一些局限性。