Dasgupta M K, Larabie M, Halloran P F
Department of Medicine, University of Alberta, Edmonton, Canada.
Kidney Int. 1994 Aug;46(2):475-81. doi: 10.1038/ki.1994.297.
As peritoneal macrophages require Interferon-gamma (IFN-gamma) for bacterial lysis, IFN-gamma levels were measured in peritoneal dialysis effluents. (PDE) by a specific radioimmunoassay. High IFN-gamma levels were found in patients with peritonitis compared to low levels in patients without peritonitis (mean 9.73 +/- 2.63 SE U/ml, N = 39 vs. 0.25 +/- 0.04, N = 32). IFN-gamma levels varied among different bacteria: Staph. aureus (highest: 23.4 +/- 5.7, N = 14), Staph. epidermidis (lower: 3.2 +/- 0.8, N = 13), other gram-positive (1.06 +/- 0.32, N = 6), gram-negative bacteria (lowest: 0.57 +/- 0.30, N = 6). After treatment of peritonitis levels decreased. In corresponding blood and PDE samples, by comparing IFN-gamma levels in 10 peritoneal dialysis patients (5 with peritonitis, 5 without), levels were raised only in PDE of patients with peritonitis, implying local IFN-gamma production. Total lymphocytes, T, B and monocyte subsets in patients' plasma and PDE did not differ, except for a higher number of mononuclear cells in PDE of patients with peritonitis (P < 0.05). Further investigation of in vitro IFN-gamma production in PDE with peritoneal monocytes, syngeneic host lymphocytes, and bacteria showed that Staph. aureus induced the highest levels of IFN-gamma and E. coli the lowest, in experiments with T cell enriched host lymphocytic fractions. We conclude that Staph. aureus peritonitis induces high levels of IFN-gamma in PDE, possibly by a T cell dependent superantigen response.
由于腹膜巨噬细胞裂解细菌需要γ干扰素（IFN-γ），因此通过特异性放射免疫测定法测量了腹膜透析流出液（PDE）中的IFN-γ水平。与无腹膜炎患者的低水平相比，腹膜炎患者中发现了高IFN-γ水平（平均9.73±2.63 SE U/ml，N = 39对0.25±0.04，N = 32）。IFN-γ水平在不同细菌之间有所不同：金黄色葡萄球菌（最高：23.4±5.7，N = 14），表皮葡萄球菌（较低：3.2±0.8，N = 13），其他革兰氏阳性菌（1.06±0.32，N = 6），革兰氏阴性菌（最低：0.57±0.30，N = 6）。腹膜炎治疗后水平下降。在相应的血液和PDE样本中，通过比较10名腹膜透析患者（5名有腹膜炎，5名无腹膜炎）的IFN-γ水平，仅腹膜炎患者的PDE中水平升高，这意味着局部IFN-γ产生。患者血浆和PDE中的总淋巴细胞、T细胞、B细胞和单核细胞亚群没有差异，除了腹膜炎患者的PDE中单核细胞数量较多（P < 0.05）。对PDE中腹膜单核细胞、同基因宿主淋巴细胞和细菌的体外IFN-γ产生的进一步研究表明，在富含T细胞的宿主淋巴细胞组分的实验中，金黄色葡萄球菌诱导的IFN-γ水平最高，大肠杆菌诱导的水平最低。我们得出结论，金黄色葡萄球菌腹膜炎可能通过T细胞依赖性超抗原反应在PDE中诱导高水平的IFN-γ。
