一种确定自由活动大鼠纹状体细胞外单单位记录的斑块-基质分区位置的方法。

A methodology for determining the patch-matrix compartmental location of extracellular single-unit recordings in the striatum of freely moving rats.

作者信息

Heidenreich B A, Trytek E S, Schroeder D M, Sengelaub D R, Rebec G V

机构信息

Department of Psychology, Indiana University, Bloomington 47405.

出版信息

J Neurosci Methods. 1994 Jun;52(2):169-74. doi: 10.1016/0165-0270(94)90127-9.

DOI:10.1016/0165-0270(94)90127-9

PMID:7967719

Abstract

A methodology was developed to combine extracellular electrophysiological recording techniques in awake, behaving rats with immunohistochemical protocols to determine the placement of recording sites in the patch (striosome) or matrix (extrastriosome) regions of the striatum. The recording system includes a 3-barrel glass micropipette, which can be used to deposit Pontamine Sky Blue to mark a small number of neurons at the recording site. Subsequent immunostaining for calbindin allows the site to be localized within the patch-matrix organization. Other dyes or neuroanatomical probes can be ejected from other barrels of the recording pipette to label afferent and efferent structures. The methodology can be applied to many brain regions, providing for integrative studies of behavior and nervous system structure and function.

摘要

已开发出一种方法，将清醒、行为活跃的大鼠的细胞外电生理记录技术与免疫组织化学方案相结合，以确定纹状体的斑块（纹小体）或基质（纹小体之外的部分）区域中记录位点的位置。该记录系统包括一个三管玻璃微吸管，可用于注入滂胺天蓝以标记记录位点处的少数神经元。随后对钙结合蛋白进行免疫染色，可使该位点在斑块 - 基质结构中定位。其他染料或神经解剖学探针可从记录吸管的其他管中喷出，以标记传入和传出结构。该方法可应用于许多脑区，为行为与神经系统结构和功能的综合研究提供支持。