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二维电泳在脑脊液蛋白质研究中的应用(作者译)

[Bidimensional electrophoresis in the study of cerebrospinal fluid protein (author's transl)].

作者信息

Michel A M, Chambon P, Soummer A M, Martin P, Bedoucha P

出版信息

Pathol Biol (Paris). 1976 Nov;24(9):593-9.

PMID:796795
Abstract

The authors report 650 bidimensional electrophoreses of the cerebrospinal fluid (CSF). All examinations were carried out after prior concentration of CSF protein. The technic used is that described by Rebeyrotte in 1970. Four types of tracing were noted: -- Two tracings were unusual by the presence of a large peak in the alpha-2 region. -- One tracing was of the hypergamma type. -- One tracing was comparable to serum by the richness of the precipitations. A change in the first migration permitted us to obtain electrophoretic separations from 100 microlitres of pure CSF. The bidimensional immunoelectrophoresis tracings are comparable to those described previously.

摘要

作者报告了650次脑脊液(CSF)的双向电泳。所有检查均在脑脊液蛋白质预先浓缩后进行。所使用的技术是1970年雷贝罗特所描述的技术。记录到四种类型的图谱:——两种图谱因在α-2区域出现一个大峰而异常。——一种图谱为高γ型。——一种图谱因沉淀丰富而与血清图谱相似。首次迁移的改变使我们能够从100微升纯脑脊液中获得电泳分离结果。双向免疫电泳图谱与先前描述的图谱相似。

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