整合宿主因子与大肠杆菌中一类独特的复杂重复基因外DNA序列结合。

Integration host factor binds to a unique class of complex repetitive extragenic DNA sequences in Escherichia coli.

作者信息

Oppenheim A B, Rudd K E, Mendelson I, Teff D

机构信息

Department of Molecular Genetics, Hebrew University, Hadassah Medical School, Jerusalem, Israel.

出版信息

Mol Microbiol. 1993 Oct;10(1):113-22. doi: 10.1111/j.1365-2958.1993.tb00908.x.

DOI:10.1111/j.1365-2958.1993.tb00908.x

PMID:7968507

Abstract

Interspersed repeated DNA sequences are characteristic features of both prokaryotic and eukaryotic genomes. REP sequences are defined as conserved repetitive extragenic palindromic sequences and are found in Escherichia coli, Salmonella typhimurium and other closely related enteric bacteria. These REP sequences may participate in the folding of the bacterial chromosome. In this work we describe a unique class of 28 conserved complex REP clusters, about 100bp long, in which two inverted REPs are separated by a singular integration host factor (IHF) recognition sequence. We term these sequences RIP (for repetitive IHF-binding palindromic) elements and demonstrate that IHF binds to them specifically. It is estimated that there are about 70 RIP elements in E. coli. Our analysis shows that the RIP elements are evenly distributed around the bacterial chromosome. The possible function of the RIP element is discussed.

摘要

散布的重复DNA序列是原核生物和真核生物基因组的特征。REP序列被定义为保守的重复基因外回文序列，存在于大肠杆菌、鼠伤寒沙门氏菌和其他密切相关的肠道细菌中。这些REP序列可能参与细菌染色体的折叠。在这项工作中，我们描述了一类独特的28个保守的复杂REP簇，长度约为100bp，其中两个反向REP被一个单一的整合宿主因子（IHF）识别序列隔开。我们将这些序列称为RIP（重复的IHF结合回文）元件，并证明IHF能特异性地结合它们。据估计，大肠杆菌中约有70个RIP元件。我们的分析表明，RIP元件在细菌染色体周围均匀分布。本文还讨论了RIP元件可能的功能。