肠杆菌科中IS1397的转座及ISKpn1的首次鉴定，ISKpn1是一种与肺炎克雷伯菌回文单元相关的新插入序列。
Transposition of IS1397 in the family Enterobacteriaceae and first characterization of ISKpn1, a new insertion sequence associated with Klebsiella pneumoniae palindromic units.
作者信息
Wilde C, Bachellier S, Hofnung M, Clément J M
机构信息
Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS URA 1444, Institut Pasteur, 75724 Paris Cedex 15, France.
出版信息
J Bacteriol. 2001 Aug;183(15):4395-404. doi: 10.1128/JB.183.15.4395-4404.2001.
Abstract
IS1397 and ISKpn1 are IS3 family members which are specifically inserted into the loop of palindromic units (PUs). IS1397 is shown to transpose into PUs with sequences close or identical to the Escherichia coli consensus, even in other enterobacteria (Salmonella enterica serovar Typhimurium, Klebsiella pneumoniae, and Klebsiella oxytoca). Moreover, we show that homologous intergenic regions containing PUs constitute IS1397 transpositional hot spots, despite bacterial interspersed mosaic element structures that differ among the three species. ISKpn1, described here for the first time, is specific for PUs from K. pneumoniae, in which we discovered it. A sequence comparison between the two insertion sequences allowed us to define a motif possibly accounting for their specificity.
摘要
IS1397和ISKpn1是IS3家族成员，它们特异性地插入到回文单元（PU）的环中。研究表明，即使在其他肠杆菌（肠炎沙门氏菌鼠伤寒血清型、肺炎克雷伯菌和产酸克雷伯菌）中，IS1397也能转座到与大肠杆菌共有序列相近或相同的PU序列中。此外，我们发现，尽管这三种细菌的细菌散布镶嵌元件结构不同，但含有PU的同源基因间区域构成了IS1397转座热点。本文首次描述的ISKpn1对我们在肺炎克雷伯菌中发现的PU具有特异性。对这两个插入序列的序列比较使我们能够确定一个可能解释其特异性的基序。
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