Cao C, Yamasaki S, Lin Z, Kurazono H, Takeda Y
Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.
Microbiol Immunol. 1994;38(6):435-40. doi: 10.1111/j.1348-0421.1994.tb01804.x.
A bead-enzyme-linked immunosorbent assay to specifically detect a Verotoxin 2 variant, VT2vp1, was developed. The sensitivity of the bead-ELISA was 200 pg/ml of the purified VT2vp1 and it did not react with 20 ng/ml of the purified VT2. The specificity of the bead-ELISA was examined with 107 strains of Verocytotoxin-producing Escherichia coli that include VT1-, VT2-, VT2vha-, VT2vhb- and VT2vp1-producing E. coli, and only VT2vp1-producing E. coli that were confirmed by VT2vp1-specific polymerase chain reaction gave positive results. It was noted that all 58 VT2vp1-producing E. coli strains were from pigs, but not from cows and humans.
开发了一种用于特异性检测维罗毒素2变体VT2vp1的珠粒酶联免疫吸附测定法。珠粒酶联免疫吸附测定法的灵敏度为纯化的VT2vp1 200 pg/ml，且不与20 ng/ml的纯化VT2发生反应。用107株产志贺毒素大肠杆菌检测了珠粒酶联免疫吸附测定法的特异性，这些菌株包括产VT1、VT2、VT2vha、VT2vhb和VT2vp1的大肠杆菌，只有经VT2vp1特异性聚合酶链反应确认的产VT2vp1的大肠杆菌给出阳性结果。值得注意的是，所有58株产VT2vp1的大肠杆菌菌株均来自猪，而非牛和人。
