Cepek K L, Shaw S K, Parker C M, Russell G J, Morrow J S, Rimm D L, Brenner M B
Department of Rheumatology and Immunology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Nature. 1994 Nov 10;372(6502):190-3. doi: 10.1038/372190a0.
In contrast to sessile cell types, lymphocytes migrate through the vasculature to become diffusely distributed in tissues or organized in lymphoid structures. A complex array of adhesion molecules including selectins, integrins and their counter-receptors mediate lymphocyte homing and migration into tissues and may be constitutively expressed or induced. However, the molecules that mediate the tissue-specific retention of lymphocytes within the parenchyma have not been identified. Along the epithelium at the basolateral surface of enterocytes, intestinal intraepithelial lymphocytes are found. These T cells of the mucosal immune system serve as a model for the tissue-specific compartmentalization of lymphocytes. We investigated whether the localization of these intestinal intraepithelial lymphocytes could be mediated by specific interactions between adhesion molecules expressed selectively on this subpopulation of T cells and tissue-restricted adhesion molecules on epithelial cells. Here we show that heterotypic adhesive interactions between epithelial cells and intraepithelial lymphocytes in vitro are mediated by E-cadherin and the alpha E beta 7 integrin.
与固着型细胞类型不同，淋巴细胞通过脉管系统迁移，从而在组织中呈弥散分布或在淋巴结构中有序排列。包括选择素、整合素及其配体在内的一系列复杂的黏附分子介导淋巴细胞归巢和向组织内的迁移，这些分子可能组成性表达或被诱导表达。然而，介导淋巴细胞在实质组织中特异性滞留的分子尚未明确。在肠上皮细胞基底外侧表面的上皮层中可发现肠道上皮内淋巴细胞。这些黏膜免疫系统的T细胞可作为淋巴细胞组织特异性分隔的模型。我们研究了这些肠道上皮内淋巴细胞的定位是否可由该T细胞亚群选择性表达的黏附分子与上皮细胞上组织限制性黏附分子之间的特异性相互作用介导。在此我们表明，体外上皮细胞与上皮内淋巴细胞之间的异型黏附相互作用由E-钙黏蛋白和αEβ7整合素介导。
