Paschen W, Dux E, Djuricic B
Max-Planck-Institute for Neurological Research, Department of Experimental Neurology, Köln, FRG.
Neurosci Lett. 1994 Jun 6;174(1):109-12. doi: 10.1016/0304-3940(94)90131-7.
The extent of RNA editing of the glutamate receptor subunit GluR5 mRNA was evaluated in the cortex, hippocampus and cerebellum of embryonic rat brains, aged 19 days, and adult brains, aged 2-3 months. RNA was isolated and transcribed into cDNA, which was used as template for amplifying a PCR product across the edited region of the GluR5 subunit. Quantification of editing was performed by restriction digest of PCR products with BbvI and image analysis of bands obtained after electrophoresis. The extent of editing was significantly lower in the embryonic state as compared to the adult state. These differences were most pronounced in the cerebellum where the extent of editing amounted to 27 +/- 8% and 78 +/- 2% in the embryonic and adult state, respectively (P < 0.001). Since expression of GluR5 is relatively high in brain areas of neuronal differentiation and synapse formation it is concluded that un-edited GluR5 may play a role in the development of the nervous system.
在19日龄的胚胎大鼠脑以及2 - 3月龄的成年大鼠脑的皮质、海马体和小脑中，评估了谷氨酸受体亚基GluR5 mRNA的RNA编辑程度。分离RNA并转录成cDNA，将其用作模板，以扩增跨越GluR5亚基编辑区域的PCR产物。通过用BbvI对PCR产物进行限制性消化以及对电泳后获得的条带进行图像分析来进行编辑程度的定量。与成年状态相比，胚胎状态下的编辑程度显著更低。这些差异在小脑中最为明显，在胚胎和成年状态下，编辑程度分别为27±8%和78±2%（P < 0.001）。由于GluR5在神经元分化和突触形成的脑区中表达相对较高，因此得出结论，未编辑的GluR5可能在神经系统发育中起作用。
