Bernard A, Khrestchatisky M
INSERM Unité 29, Paris, France.
J Neurochem. 1994 May;62(5):2057-60. doi: 10.1046/j.1471-4159.1994.62052057.x.
Kainate (KA) is a potent neuroexcitatory agent that induces seizure and brain damage syndromes with increasing efficiency during maturation. It has been suggested that the selective neuronal damage induced by KA may result not only from its depolarizing actions, but also from intracellular accumulation of Ca2+. The effects of KA are mediated by specific high-affinity receptors, enriched in the hippocampus. Members of this class of receptors, GluR5 and GluR6, have been characterized by cDNA cloning. Ca2+ permeability of the GluR6 receptor is determined by editing in the corresponding RNA. We report here a rapid PCR-based approach to assess in all experimental conditions the levels of GluR5 and GluR6 editing in the transmembrane TMII region. We show that editing in both GluR5 and GluR6 RNA is developmentally regulated and that different regions of the adult rat hippocampus demonstrate distinct levels of GluR6 editing.
海人酸（KA）是一种强效神经兴奋剂，在成熟过程中，它诱发癫痫和脑损伤综合征的效率越来越高。有人提出，KA诱导的选择性神经元损伤可能不仅源于其去极化作用，还源于细胞内Ca2+的积累。KA的作用由特定的高亲和力受体介导，这些受体在海马体中富集。这类受体的成员，即GluR5和GluR6，已通过cDNA克隆进行了表征。GluR6受体的Ca2+通透性由相应RNA中的编辑决定。我们在此报告一种基于PCR的快速方法，用于在所有实验条件下评估跨膜TMII区域中GluR5和GluR6的编辑水平。我们表明，GluR5和GluR6 RNA中的编辑均受发育调控，并且成年大鼠海马体的不同区域表现出不同水平的GluR6编辑。
