Li S, Pelletier G
MRC Group in Molecular Endocrinology, CHUL Research Center, Québec, Canada.
Neurosci Lett. 1994 Jun 6;174(1):61-3. doi: 10.1016/0304-3940(94)90119-8.
To verify the hypothesis that GnRH neurons can influence each other, we have studied the effects of GnRH, a potent GnRH agonist (Des-Gly10, D-Ala6-proethylamide9)-GnRH and a GnRH antagonist Antide on GnRH gene expression in the male rat preoptic area (POA). Hypophysectomized rats received into the left lateral brain ventricle either the vehicle or each of the peptides. GnRH mRNA levels in neurons in POA were evaluated by quantitative in situ hybridization using a 35S-labelled 48-base oligonucleotide. In hypophysectomized animals, a 18% decrease in the number of silver grain overlying GnRH neurons was observed as compared to intact animals. In hypophysectomized animals neither GnRH nor the GnRH agonist modified GnRH mRNA levels. On the other hand, the administration of Antide induced a 34% increase in the levels of GnRH mRNA. These results suggest that GnRH exerts a negative regulation of the GnRH gene expression in neurons in the POA probably via GnRH receptors.
为了验证促性腺激素释放激素（GnRH）神经元能够相互影响这一假说，我们研究了GnRH、一种强效GnRH激动剂（去甘氨酸10、D-丙氨酸6-脯氨酰胺9）-GnRH以及一种GnRH拮抗剂Antide对雄性大鼠视前区（POA）中GnRH基因表达的影响。垂体切除的大鼠通过左侧脑室注射溶剂或每种肽。使用35S标记的48碱基寡核苷酸，通过定量原位杂交评估POA中神经元的GnRH mRNA水平。与完整动物相比，垂体切除的动物中，覆盖GnRH神经元的银颗粒数量减少了18%。在垂体切除的动物中，GnRH和GnRH激动剂均未改变GnRH mRNA水平。另一方面，给予Antide可使GnRH mRNA水平升高34%。这些结果表明，GnRH可能通过GnRH受体对POA中神经元的GnRH基因表达发挥负调控作用。
