Kawakami F, Okamura H, Inatomi T, Tamada Y, Nakajima T, Ibata Y
Department of Psychiatry, Kyoto Prefectural University of Medicine, Japan.
Neurosci Lett. 1994 Jun 6;174(1):81-4. doi: 10.1016/0304-3940(94)90124-4.
The influence of serotonergic inputs on the levels of vasoactive intestinal peptide (VIP) mRNA in the rat suprachiasmatic nucleus (SCN) was examined by in situ hybridization combined with morphometrical analysis. Depletion of serotonin by p-chlorophenylalanine methyl ester (300 mg/kg i.p. daily for 3 days) caused a marked decrease in VIP mRNA signal levels in the ventrolateral part of the SCN. This finding suggests that serotonergic input controls VIP production at the mRNA level.
通过原位杂交结合形态计量分析，研究了5-羟色胺能输入对大鼠视交叉上核（SCN）中血管活性肠肽（VIP）mRNA水平的影响。用对氯苯丙氨酸甲酯（每天腹腔注射300mg/kg，连续3天）耗尽5-羟色胺，导致SCN腹外侧部分的VIP mRNA信号水平显著降低。这一发现表明，5-羟色胺能输入在mRNA水平上控制VIP的产生。
