Warembourg M, Deneux D, Jolivet A
INSERM U 156, Lille, France.
Neurosci Lett. 1994 Jun 6;174(1):89-92. doi: 10.1016/0304-3940(94)90126-0.
Double-label immunocytochemistry has been employed to determine whether progesterone receptor (PR)-containing neurons in the arcuate nucleus (AR) of female guinea pig contain neuropeptide Y (NPY). Animals were ovariectomized, primed by estradiol and microinjected intracerebroventricularly with colchicine to visualize NPY-immunoreactive (IR) neurons. Only a very small population of NPY-IR perikarya (2.5% approximately) were found to be also PR-IR. NPY-IR varicosities appeared in close proximity to neurons with PR-containing nuclei. This restricted coexistence provides morphological evidence that most NPY neurons in the AR are not directly regulated by progesterone.
