Sato A, Uchida S, Yamauchi Y
Department of the Autonomic Nervous System, Tokyo Metropolitan Institute of Gerontology, Japan.
Neurosci Lett. 1994 Jul 4;175(1-2):149-52. doi: 10.1016/0304-3940(94)91101-0.
We have developed a new system to continuously measure regional cerebral blood flow (rCBF) in the cortex of a conscious animal. For this purpose, we used rats and laser Doppler flowmetry. Under pentobarbital anesthesia, the animal's skull was opened making a small square hole 3 mm x 3 mm in size. A transparent acrylic plate was placed over the hole in the skull. A polyethylene cannula (inner diameter 1.0 mm, length 5.0 mm) was fixed on the plate as a guide for the laser Doppler flowmeter (LDF) probe (outer diameter 1.0 mm, length 5.5 mm). Both the plate and guide cannula were fixed to the skull by dental cement. Every day for the following two weeks after surgery, the conscious animal was placed in a hammock for recording rCBF. A LDF probe was freely attachable to the plate above the cortex via the guide cannula during measurement of rCBF. The rats were kept in a hammock with their legs firmly touching the floor during measurement of rCBF. It was possible to measure rCBF every day for about two weeks, and rCBF responded consistently to inhalation of 7% CO2 when the responses were expressed as percentages of the prestimulus control rCBF values. This system is recommended for the continuous measurement of rCBF in a conscious animal.
我们开发了一种新系统，用于连续测量清醒动物皮质中的局部脑血流量（rCBF）。为此，我们使用了大鼠和激光多普勒血流仪。在戊巴比妥麻醉下，打开动物的颅骨，形成一个3毫米×3毫米的小方孔。在颅骨上的孔上放置一块透明丙烯酸板。将一根聚乙烯套管（内径1.0毫米，长度5.0毫米）固定在板上，作为激光多普勒血流仪（LDF）探头（外径1.0毫米，长度5.5毫米）的导向装置。板和导向套管都用牙科粘固剂固定在颅骨上。在手术后的接下来两周里，每天将清醒的动物放在吊床上记录rCBF。在测量rCBF期间，LDF探头可通过导向套管自由连接到皮质上方的板上。在测量rCBF期间，将大鼠放在吊床上，使其腿部牢固地接触地面。在大约两周的时间里，每天都可以测量rCBF，并且当将反应表示为刺激前对照rCBF值的百分比时，rCBF对吸入7% CO2的反应一致。该系统推荐用于清醒动物rCBF的连续测量。
