Bagetta G, Finazzi-Agrò A, Palma E, Nisticò G
Faculty of Pharmacy, Calabria University, Cosenza, Italy.
Neurosci Lett. 1994 Jul 18;176(1):97-100. doi: 10.1016/0304-3940(94)90880-x.
The human immunodeficiency virus type 1 (HIV-1) coat glycoprotein, GP120 has been reported to cause death of several neuronal cell types maintained in vitro. In the present experiments the gross behavioural, electrocortical (ECoG) and neuropathological effects of GP120 were studied in rats chronically microinfused into one lateral cerebral ventricle (i.c.v.) via mini-osmotic pumps. Treatment with GP120 (100 ng/day) for 1, 7 and 14 consecutive days lacked postural, motor and ECoG effects nor did it produce any apparent brain damage. In addition, acute i.c.v. injection of a subconvulsive dose (500 ng) of N-methyl-D-aspartate (NMDA) did not produce motor and ECoG epileptogenic discharges in rats which received 1 h beforehand a dose of GP120 (900 ng) into the dorsal hippocampus ipsilateral to the injected ventricle; per se this dose of GP120 was ineffective. In conclusion, the present experiments demonstrate that acute or chronic microinfusion of GP120 into the rat cerebral ventricular system does not produce neurotoxic effects. In addition, they demonstrate that intrahippocampal GP120 does not sensitize rats to the excitotoxic effects of a subconvulsive dose of NMDA.
据报道，1型人类免疫缺陷病毒（HIV-1）包膜糖蛋白GP120可导致多种体外培养的神经元细胞死亡。在本实验中，通过微型渗透泵将GP120长期微量注入大鼠一侧侧脑室（脑室内），研究其对大鼠整体行为、皮层脑电图（ECoG）和神经病理学的影响。连续1天、7天和14天用GP120（100纳克/天）治疗，未产生姿势、运动和ECoG方面的影响，也未造成任何明显的脑损伤。此外，在预先向注射脑室同侧背侧海马注射一剂GP120（900纳克）1小时后的大鼠中，急性脑室内注射亚惊厥剂量（500纳克）的N-甲基-D-天冬氨酸（NMDA）未产生运动和ECoG癫痫样放电；就其本身而言，该剂量的GP120无效。总之，本实验表明，向大鼠脑室系统急性或慢性微量注入GP120不会产生神经毒性作用。此外，实验还表明，海马内注射GP120不会使大鼠对亚惊厥剂量的NMDA的兴奋毒性作用敏感。
