Imayama S, Furumura M, Hori Y
Department of Dermatology, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Pigment Cell Res. 1994 Jun;7(3):170-4. doi: 10.1111/j.1600-0749.1994.tb00045.x.
Scanning electron microscopy with immunogold labeling was used to demonstrate the in vivo distribution of molecules of basic fibroblast growth factor (bFGF) that were expressed and/or present on the surface of the cells of the normal epidermis and dermal connective tissue of humans. We found that molecules of bFGF, seen as deposits of gold particles, were present densely on the surfaces of the melanocytes but not the epidermal keratinocytes. In connective tissue, these molecules were present exclusively on the surfaces of the fibroblasts, macrophages, vascular endothelial cells, and the basement membrane surrounding the endothelial tube. The selective deposition of bFGF molecules on the melanocytes suggests that the dermal connective tissue may be involved in controlling the proliferation of melanocytes by means of bFGF molecules in vivo, since these melanocytes require bFGF to proliferate in vitro. The latter is synthesized and stored exclusively in the connective tissue.
采用免疫金标记扫描电子显微镜来展示碱性成纤维细胞生长因子（bFGF）分子在人类正常表皮细胞和真皮结缔组织细胞表面的体内分布情况。我们发现，被视为金颗粒沉积物的bFGF分子密集地存在于黑素细胞表面，而不存在于表皮角质形成细胞表面。在结缔组织中，这些分子仅存在于成纤维细胞、巨噬细胞、血管内皮细胞以及围绕内皮管的基底膜表面。bFGF分子在黑素细胞上的选择性沉积表明，真皮结缔组织可能在体内通过bFGF分子参与控制黑素细胞的增殖，因为这些黑素细胞在体外增殖需要bFGF。后者仅在结缔组织中合成和储存。
