Shirley M W
AFRC Institute for Animal Health, Compton Laboratory, Nr Newbury, Berks, UK.
Parasitol Res. 1994;80(5):366-73. doi: 10.1007/BF00932373.
The molecular karyotype of the coccidial parasite Eimeria tenella has been further resolved. Different conditions of pulsed-field gel electrophoresis were used to separate up to 14 chromosomes that ranged in size between 1 and in excess of 6 megabase pairs (Mb) of DNA. Comparisons of several populations revealed some size polymorphisms of homologous chromosomes. Probes containing tandem repeats of the DNA sequence CAG and the telomeric-like sequence TTTAGGG hybridised to each chromosome. All other probes containing either repetitive or non-repetitive sequences were mapped to individual chromosomes.
球虫寄生虫柔嫩艾美耳球虫的分子核型已得到进一步解析。采用不同条件的脉冲场凝胶电泳分离出多达14条染色体，其大小在1至超过6兆碱基对（Mb）的DNA之间。对几个群体的比较揭示了同源染色体的一些大小多态性。含有DNA序列CAG串联重复序列和端粒样序列TTTAGGG的探针与每条染色体杂交。所有其他含有重复或非重复序列的探针都被定位到单个染色体上。
