Bevec D, Klier H, Holter W, Tschachler E, Valent P, Lottspeich F, Baumruker T, Hauber J
Sandoz Research Institute, Vienna, Austria.
Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):10829-33. doi: 10.1073/pnas.91.23.10829.
The hypusine-containing protein eukaryotic initiation factor 5A (eIF-5A) is a cellular cofactor critically required for the function of the Rev transactivator protein of human immunodeficiency virus type 1 (HIV-1). eIF-5A localizes in the nuclear and cytoplasmic compartments of mammalian cells, suggesting possible activities on the level of regulated mRNA transport and/or protein translation. In this report we show that eIF-5A gene expression is constitutively low but inducible with T-lymphocyte-specific stimuli in human peripheral blood mononuclear cells (PBMCs) of healthy individuals. In contrast, eIF-5A is constitutively expressed at high levels in human cell lines as well as in various human organs. Comparison of eIF-5A levels in the PBMCs of uninfected and HIV-1-infected donors shows a significant upregulation of eIF-5A gene expression in the PBMCs of HIV-1 patients, compatible with a possible role of eIF-5A in HIV-1 replication during T-cell activation.
含hypusine的蛋白质真核生物起始因子5A（eIF-5A）是一种细胞辅因子，对于人类免疫缺陷病毒1型（HIV-1）的Rev反式激活蛋白的功能至关重要。eIF-5A定位于哺乳动物细胞的细胞核和细胞质区室，提示其可能在调控mRNA转运和/或蛋白质翻译水平发挥作用。在本报告中，我们表明eIF-5A基因表达在健康个体的人外周血单个核细胞（PBMC）中本底水平较低，但可被T淋巴细胞特异性刺激诱导。相比之下，eIF-5A在人类细胞系以及各种人体器官中本底高水平表达。未感染和HIV-1感染供体的PBMC中eIF-5A水平的比较显示，HIV-1患者的PBMC中eIF-5A基因表达显著上调，这与eIF-5A在T细胞激活期间HIV-1复制中可能发挥的作用相符。
