Koettnitz K, Kappel B, Baumruker T, Hauber J, Bevec D
SANDOZ Research Institute, Vienna, Austria.
Gene. 1994 Jul 8;144(2):249-52. doi: 10.1016/0378-1119(94)90385-9.
A genomic clone encoding the human eukaryotic initiation factor 5A (eIF-5A) was isolated and its entire nucleotide sequence was determined. The whole eIF-5A coding region is not interrupted by introns. The functional eIF-5A gene is highly homologous to the corresponding complementary DNA. One single 1.4-kb transcript thereof is expressed in human cell lines. Furthermore, we also isolated and sequenced two additional eIF-5A-related sequences which are, by expression and sequence analyses, identified as pseudogenes of the functional eIF-5A. The sequence homology between these pseudogenes and the functional eIF-5A is 71 and 87%.
分离出了一个编码人真核生物起始因子5A（eIF-5A）的基因组克隆，并测定了其完整的核苷酸序列。整个eIF-5A编码区没有被内含子打断。功能性eIF-5A基因与相应的互补DNA高度同源。其一个单一的1.4kb转录本在人细胞系中表达。此外，我们还分离并测序了另外两个与eIF-5A相关的序列，通过表达和序列分析，它们被鉴定为功能性eIF-5A的假基因。这些假基因与功能性eIF-5A之间的序列同源性分别为71%和87%。
