Zhang X, Bao L, Xu Z Q, Kopp J, Arvidsson U, Elde R, Hökfelt T
Department of Neuroscience, Karolinska Institute, Stockholm, Sweden.
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11738-42. doi: 10.1073/pnas.91.24.11738.
Immunohistochemical staining with an antiserum against the neuropeptide Y (NPY)-Y1 receptor (Y1-R) protein was shown in rat small dorsal root ganglion (DRG) neurons, which also were Y1-R mRNA-positive and calcitonin gene-related peptide (CGRP)-positive. The Y1-R-like immunoreactivity was almost exclusively located in the somatic plasmalemma and in the perinuclear region. Intracellular recording showed that the Y1 agonist [Leu31,Pro34]NPY evoked an outward current in small DRG neurons, suggesting a functional somatic Y1-R. No evidence for axonal transport of Y1-R protein was obtained after analysis of the dorsal horn for double staining with CGRP, after dorsal rhizotomy, or after compression of dorsal roots and the sciatic nerve. It is proposed that blood-borne NPY released from sympathetic nerves and adrenal medulla is the endogenous ligand for the Y1 receptors on the small DRG neurons. Y1-R-positive neurons were also seen in the dorsal horn of spinal cord, the hypothalamic arcuate nucleus, pyramidal cells in the cerebral cortex, Purkinje and basket cells in the cerebellar cortex, and in many other brain regions.
用抗神经肽Y（NPY）-Y1受体（Y1-R）蛋白的抗血清进行免疫组织化学染色显示，大鼠小背根神经节（DRG）神经元呈阳性，这些神经元Y1-R mRNA和降钙素基因相关肽（CGRP）也呈阳性。Y1-R样免疫反应几乎仅位于体细胞质膜和核周区域。细胞内记录显示，Y1激动剂[Leu31，Pro34]NPY在小DRG神经元中诱发外向电流，提示存在功能性的体细胞Y1-R。在用CGRP进行双重染色分析背角、背根切断术后或背根和坐骨神经受压后，均未获得Y1-R蛋白轴突运输的证据。有人提出，交感神经和肾上腺髓质释放的血源性NPY是小DRG神经元上Y1受体的内源性配体。在脊髓背角、下丘脑弓状核、大脑皮质锥体细胞、小脑皮质浦肯野细胞和篮状细胞以及许多其他脑区也可见到Y1-R阳性神经元。
