Igbo I N, Reigel C E, Greene I M, Kenny A D
Department of Pharmacology, Texas Tech University Health Sciences Center, Lubbock 79430.
Pharmacology. 1994 Aug;49(2):112-20. doi: 10.1159/000139223.
We studied the action of beta-adrenergic agonists on Japanese quail erythrocyte carbonic anhydrase (CA) in vitro. Earlier we had reported that epinephrine increased CA activity by 14%; the present study focused on an attempt to increase the size of this response. Washed erythrocytes from reserpine-treated (1 mg/kg daily i.m. for 3 days) and control birds were incubated for 40 min in the presence of isoproterenol 10(-6) mol/l. The activity of CA expressed as Wilbur-Anderson units/mg hemoglobin was increased by as much as 42% in reserpine-treated birds over the control depending on the conditions. Addition of 10(-5) mol/l of the beta-adrenergic antagonist propranolol inhibited the isoproterenol-induced effect in nonreserpinized birds. We conclude that pretreatment with reserpine, which was accompanied by a fall in plasma catecholamine levels, particularly epinephrine levels, enhanced the activation of CA by isoproterenol.
我们在体外研究了β-肾上腺素能激动剂对日本鹌鹑红细胞碳酸酐酶（CA）的作用。早些时候我们报道肾上腺素可使CA活性增加14%；本研究着重尝试增大这种反应的幅度。将经利血平处理（每天肌肉注射1mg/kg，共3天）的鹌鹑和对照鹌鹑的洗涤红细胞，在10⁻⁶mol/L异丙肾上腺素存在的情况下孵育40分钟。根据条件不同，以威尔伯-安德森单位/毫克血红蛋白表示的CA活性，在经利血平处理的鹌鹑中比对照鹌鹑增加了多达42%。添加10⁻⁵mol/L的β-肾上腺素能拮抗剂普萘洛尔可抑制未用利血平处理的鹌鹑中异丙肾上腺素诱导的效应。我们得出结论，利血平预处理伴随着血浆儿茶酚胺水平尤其是肾上腺素水平的下降，增强了异丙肾上腺素对CA的激活作用。
