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[使用“微型弯曲菌检测法”对弯曲菌进行生化特性分析]

[Biochemical characterization of Campylobacter using the "Micro Campy" method].

作者信息

Granados Silvestre M A, López-Vidal Y, Calva M J J, Guerrero Almeida M L

机构信息

Departamento de Infectología, Instituto Nacional de la Nutrición Salvador Zubirán, México, D.F.

出版信息

Rev Invest Clin. 1994 May-Jun;46(3):221-9.

PMID:7973146
Abstract

Three species of Campylobacter are recognized as enteropathogens in children under five years of age and in immunocompromised patients. Several groups have tried to classify them using biochemical profiles and have found six different patterns which comprise 12 individual tests. We have designed a "Micro Campy" method which identifies C. jejuni, C. coli and C. lari classifying them into several biotypes. Fifty two Campylobacter strains isolated from humans were studied and classified in 24 different patterns. Strains isolated from diarrhea episodes were seen in two specific patterns. We propose the use of this easy to perform method which has a reproducibility higher than 92% in biotyping Campylobacter strains.

摘要

三种弯曲杆菌被认为是五岁以下儿童和免疫功能低下患者的肠道病原体。有几个研究小组试图利用生化特征对它们进行分类,发现了六种不同的模式,这些模式包含12项独立测试。我们设计了一种“微量弯曲杆菌”方法,该方法可识别空肠弯曲杆菌、结肠弯曲杆菌和拉氏弯曲杆菌,并将它们分为几种生物型。对从人类分离出的52株弯曲杆菌菌株进行了研究,并将其分为24种不同的模式。从腹泻病例中分离出的菌株呈现出两种特定模式。我们建议使用这种易于操作的方法,该方法在对弯曲杆菌菌株进行生物分型时的重现性高于92%。

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