Nosti-Palacios R, Terrazas-Valdés L I, García-Tamayo F
Unidad de Investigaciones en Salud Infantil del Instituto Nacional de Pediatría, México, D.F., Mexico.
Rev Latinoam Microbiol. 1994 Apr-Jun;36(2):113-8.
This article describes the enterobacterial common antigen (ECA) content in the liver and spleen extracts from immunosuppressed two months old CFW mice which received a single dose (400 mg/kg body weight) of cyclophosphamide (CY). A complement fixation inhibition assay was used to measure the tissular concentration of ECA. The results showed that liver and spleen ethanol-soluble extracts from immunosuppressed mice had a greater quantity of ECA than those obtained from healthy mice (p < 0.001). Previous works have proven that ECA is a hapten shared by almost all Gram-negative bacteria, and CY is an alkylating agent useful to induce bacterial translocation. Therefore, the increments observed in tissular ECA concentration may indicate the extension of the enterobacterial dissemination from intestine to abdominal organs in CY-treated immunosuppressed mice.
本文描述了接受单剂量（400毫克/千克体重）环磷酰胺（CY）的两个月大免疫抑制CFW小鼠肝脏和脾脏提取物中的肠杆菌共同抗原（ECA）含量。采用补体结合抑制试验来测定组织中ECA的浓度。结果显示，免疫抑制小鼠的肝脏和脾脏乙醇可溶性提取物中的ECA含量高于健康小鼠（p<0.001）。先前的研究已证实，ECA是几乎所有革兰氏阴性菌共有的半抗原，而CY是一种可用于诱导细菌易位的烷基化剂。因此，观察到的组织中ECA浓度的增加可能表明在接受CY治疗的免疫抑制小鼠中，肠杆菌从肠道扩散到腹部器官的范围扩大。
