Hirakawa M, Tamura A, Nagashima H, Nakayama H, Sano K
Department of Neurosurgery, Teikyo University School of Medicine, Tokyo, Japan.
Stroke. 1994 Dec;25(12):2471-5. doi: 10.1161/01.str.25.12.2471.
The purpose of this study was to investigate the behavioral changes, in particular retention of memory, after focal cerebral ischemia in rats.
Ischemia was produced by permanent occlusion of the left middle cerebral artery (MCA). For quantitative behavioral analysis, one-trial passive avoidance response and active avoidance response with the discrete lever-press avoidance procedure were observed. One group of animals was trained once to learn the passive avoidance task 1 day before surgery. The response latency was examined 4 and 14 days after surgery. The second group was trained to learn the active avoidance task for 2 weeks before surgery. The avoidance rate was examined 3 and 14 days after surgery.
The MCA-occluded group showed significant failure of memory retention in both of these tasks (P < .01). The nonoperated group and sham-operated group showed no definite memory failure.
Retention of memory in the passive avoidance response and the active avoidance response was disturbed after left MCA occlusion in the rat. These results strongly suggest that this model can be used to assess memory disturbance after focal cerebral ischemia.
本研究旨在探讨大鼠局灶性脑缺血后的行为变化，尤其是记忆保持情况。
通过永久性结扎左大脑中动脉（MCA）制造缺血模型。为进行定量行为分析，观察了一次性被动回避反应和采用离散杠杆按压回避程序的主动回避反应。一组动物在手术前1天接受一次训练以学习被动回避任务。在手术后4天和14天检查反应潜伏期。第二组动物在手术前接受2周的主动回避任务训练。在手术后3天和14天检查回避率。
MCA结扎组在这两项任务中均表现出明显的记忆保持失败（P <.01）。未手术组和假手术组未表现出明确的记忆失败。
大鼠左MCA结扎后，被动回避反应和主动回避反应中的记忆保持受到干扰。这些结果强烈表明该模型可用于评估局灶性脑缺血后的记忆障碍。
