Annesley T M, Clayton L T
Department of Pathology, University of Michigan Medical Center, Ann Arbor.
Ther Drug Monit. 1994 Aug;16(4):419-24. doi: 10.1097/00007691-199408000-00014.
A rapid and sensitive high-performance liquid chromatographic method for the analysis of the antiepileptic felbamate in human serum has been developed. Following protein precipitation with acetonitrile containing the internal standard alphenal, the drugs are extracted from serum using dichloromethane. The extraction efficiency averages 98% for felbamate. Chromatography is performed with use of reversed phase octyl (C8) column, monitored at 210 nm. The isocratic mobile phase is acetonitrile:acetate buffer, pH 5.3, flow rate 2.0 ml/min. Felbamate is resolved from other common antiepileptic drugs that may be co-administered. The assay is linear to 200 micrograms/ml in serum. The effect of mobile phase pH changes on the chromatographic characteristics of the antiepileptic drugs and some potential interferents is discussed.
已开发出一种快速灵敏的高效液相色谱法，用于分析人血清中的抗癫痫药物非氨酯。在用含内标阿尔非那的乙腈进行蛋白质沉淀后，用二氯甲烷从血清中提取药物。非氨酯的提取效率平均为98%。使用反相辛基（C8）柱进行色谱分析，在210nm波长处监测。等度流动相为乙腈：pH5.3的醋酸盐缓冲液，流速为2.0ml/min。非氨酯可与其他可能同时给药的常见抗癫痫药物分离。该测定法在血清中线性范围为200μg/ml。讨论了流动相pH变化对抗癫痫药物和一些潜在干扰物色谱特性的影响。
