高效液相色谱法同时测定人血浆中的非氨酯及其三种代谢物

Simultaneous determination of felbamate and three metabolites in human plasma by high-performance liquid chromatography.

作者信息

Romanyshyn L A, Wichmann J K, Kucharczyk N, Sofia R D

机构信息

Department of Biochemistry, Wallace Laboratories, Cranbury, New Jersey 08512.

出版信息

Ther Drug Monit. 1994 Feb;16(1):83-9. doi: 10.1097/00007691-199402000-00014.

DOI:10.1097/00007691-199402000-00014

PMID:8160261

Abstract

An isocratic liquid-chromatographic method employing one extraction step and a 4.6 mm x 150 mm Spherisorb ODS2, 3 microns high-performance liquid chromatography (HPLC) column using ultraviolet (UV)-absorbance detection at 210 nm has been developed for quantitation of felbamate (FBM) and three felbamate metabolites in 0.100-ml aliquots of human plasma. The linear quantitation range for FBM and the two hydroxy metabolites is 0.781-200 micrograms/ml, and that for the monocarbamate metabolite is 0.391-200 micrograms/ml.

摘要

已开发出一种等度液相色谱法，该方法采用一步萃取，并使用一根4.6毫米×150毫米、粒径为3微米的Spherisorb ODS2高效液相色谱（HPLC）柱，在210纳米处进行紫外（UV）吸光度检测，用于定量测定0.100毫升人血浆等分试样中的非氨酯（FBM）和三种非氨酯代谢物。FBM和两种羟基代谢物的线性定量范围为0.781 - 200微克/毫升，单氨基甲酸酯代谢物的线性定量范围为0.391 - 200微克/毫升。