Berger B J, Fairlamb A H
Department of Medical Parasitology, London School of Hygiene and Tropical Medicine, UK.
Trans R Soc Trop Med Hyg. 1994 May-Jun;88(3):357-9. doi: 10.1016/0035-9203(94)90117-1.
A sensitive high-performance liquid chromatographic method has been developed for the separation and quantitative estimation of melaminophenyl arsenical drugs used in the treatment of human and veterinary trypanosomiases. Five clinically relevant compounds (melarsoprol, melarsonyl potassium, cymelarsan, melarsen oxide, and sodium melarsen) can be separated with an octadecylsilane reverse-phase column and detected down to 10 pmol per injection. The chromatographic system utilizes a propanol gradient in water, with lithium camphor sulphonate as ion modifier, and detection by ultraviolet absorbance at 280 nm. Preliminary results indicate that the arsenical compounds can be extracted and concentrated from serum using octadecylsilane solid-phase extraction cartridges. This method represents the first specific and sensitive assay system for separating and quantitatively estimating this important class of antiparasitic agents.
已开发出一种灵敏的高效液相色谱法，用于分离和定量测定用于治疗人和兽类锥虫病的氨基苯胂药物。使用十八烷基硅烷反相柱可分离出五种临床相关化合物（美拉胂醇、美拉胂钾、赛米胂、氧化美拉胂和美拉胂钠），每次进样的检测限低至10皮摩尔。该色谱系统采用水中的丙醇梯度，以樟脑磺酸锂作为离子改性剂，并通过在280nm处的紫外吸收进行检测。初步结果表明，可使用十八烷基硅烷固相萃取柱从血清中提取和浓缩胂化合物。该方法是用于分离和定量测定这类重要抗寄生虫剂的首个特异性灵敏分析系统。
