Zhu X, Imamura M, Tanaka J, Han C W, Hashino S, Imai K, Asano M, Nakane A, Minagawa T, Kobayashi M
Third Department of Internal Medicine, Hokkaido University School of Medicine, Sapporo, Japan.
Transplantation. 1994 Nov 27;58(10):1104-9.
Reverse transcriptase-polymerase chain reaction showed that interleukin 3, IL-4, IL-5, IL-6, interferon-gamma and stem cell factor mRNA expression were higher in 15-deoxyspergualin-treated spleen cells than in control spleen cells. Increased IL-2 and IFN-gamma mRNA expression were observed in 15-deoxyspergualin-treated bone marrow cells. On the other hand, increased platelet counts in BALB/c-->C3H/He bone marrow chimeras were observed from days 20 to 33 in our previous work, when they were treated with 15-deoxyspergualin from days 14 to 25. In contrast, marked leukocytopenia and anemia were simultaneously observed, although a marked leukocytosis and a rapid recovery of anemia were observed on day 33 and thereafter. To analyze effects of 15-deoxyspergualin on hematopoiesis and the immune system, we examined mRNA expression in bone marrow and spleen cells from BALB/c-->C3H/He bone marrow chimeras treated with 15-deoxyspergualin from days 14 to 25. Reverse transcriptase-polymerase chain reaction showed that IL-3, IL-4, IL-6, stem cell factor, granulocyte colony-stimulating factor, and granulocyte/macrophage colony-stimulating factor mRNA expression were higher in 15-deoxyspergualin-treated chimeras than in control chimeras, indicating that these cytokines are responsible for an enhancement of hematopoiesis. It was conceivable that IL-6 supported thrombopoiesis in concert with other cytokines. On the contrary, increased IFN-gamma, IL-2, IL-3, IL-4, and IL-10 mRNA expression may play an immunosuppressive role in vivo.
逆转录聚合酶链反应显示，与对照脾细胞相比，15-去氧精胍菌素处理的脾细胞中白细胞介素3、IL-4、IL-5、IL-6、干扰素-γ和干细胞因子mRNA表达更高。在15-去氧精胍菌素处理的骨髓细胞中观察到IL-2和IFN-γ mRNA表达增加。另一方面，在我们之前的研究中，从第20天到第33天，观察到BALB/c→C3H/He骨髓嵌合体的血小板计数增加，此时它们从第14天到第25天用15-去氧精胍菌素处理。相反，同时观察到明显的白细胞减少和贫血，尽管在第33天及之后观察到明显的白细胞增多和贫血的快速恢复。为了分析15-去氧精胍菌素对造血和免疫系统的影响，我们检测了从第14天到第25天用15-去氧精胍菌素处理的BALB/c→C3H/He骨髓嵌合体的骨髓和脾细胞中的mRNA表达。逆转录聚合酶链反应显示，与对照嵌合体相比，15-去氧精胍菌素处理的嵌合体中IL-3、IL-4、IL-6、干细胞因子、粒细胞集落刺激因子和粒细胞/巨噬细胞集落刺激因子mRNA表达更高，表明这些细胞因子负责增强造血。可以想象，IL-6与其他细胞因子协同支持血小板生成。相反，IFN-γ、IL-2、IL-3、IL-4和IL-10 mRNA表达增加可能在体内发挥免疫抑制作用。
