[The role of Kupffer cells in regulating protein synthesis in hepatocytes].

Importance of nonparenchymal liver cells for regulation of protein synthesis in hepatocytes was studied using liver cell culture and co-culture. The rate of protein synthesis was increased in hepatocytes during simultaneous cultivation of these cells with nonparenchymal elements. Activation of protein synthesis was distinctly higher in hepatocytes co-cultivated with nonparenchymal liver cells isolated from liver tissue of rats stimulated by bacterial lipopolysaccharide. Similar results were obtained after cultivation of hepatocytes in the conditioned medium containing nonparenchymal liver cells treated with lipopolysaccharide, high density lipoprotein (HDL) and hydrocortisone. The data obtained suggest that nonparenchymal liver cells, especially Kupffer cells, contributed to regulation of protein synthesis in hepatocytes. The cooperative effect of HDL and glucocorticoids in stimulation of the mononuclear phagocytes system appears to be mainly responsible for augmented expression of genes in hepatocytes.