Pestova M I, Gmoshinskiĭ I V, Mazo V K
Vopr Pitan. 1993 May-Jun(3):41-6.
Guinea pigs were fed for 14 days with semisynthetic diets containing protein (milk casein: 1.5, 30, 45% and soya protein: 4, 18 and 36% of total energy) or with different amounts of L-methionine (Met) and glycine (Gly). Simultaneously with diets feeding the animals were sensitized epicutaneously by formaldehyde (F). The sensitization to F was evaluated by leukocyte specific lysis reaction (RSLL). Hepatic formaldehyde dehydrogenase (FDH) activity and reduced glutathione (GSH) content were measured. The sensitization to F decreased when the animals were fed with growing amount of plant or animal protein, or with high amount of Met and normal level of Gly. FDH activity increased in sensitized animals compared to non-sensitized irrespective of the diet fed. Hepatic GSH pool significantly grew with an increase in both proteins level and in sensitized animals also with growing Met and Gly intake. GSH pool did not change in sensitized animals fed with different amounts of amino acids. It is suggested that high dietary protein and amino acids protection against F-sensitization was mediated by elevated tissue GSH though optimal consumptions of Met and Gly were different. Hepatic FGH activity together with RSSL may be considered as a sensitive biochemical criterion of experimental F sensitization.
用含蛋白质（酪蛋白：占总能量的1.5%、30%、45%，大豆蛋白：占总能量的4%、18%、36%）的半合成饲料或不同量的L-蛋氨酸（Met）和甘氨酸（Gly）喂养豚鼠14天。在喂食饲料的同时，通过甲醛（F）对动物进行表皮致敏。通过白细胞特异性裂解反应（RSLL）评估对F的致敏性。测量肝脏甲醛脱氢酶（FDH）活性和还原型谷胱甘肽（GSH）含量。当给动物喂食越来越多的植物或动物蛋白，或高量的Met和正常水平的Gly时，对F的致敏性降低。与未致敏动物相比，无论喂食何种饲料，致敏动物的FDH活性均增加。肝脏GSH储备随着蛋白质水平的增加而显著增加，在致敏动物中，随着Met和Gly摄入量的增加也会增加。在喂食不同量氨基酸的致敏动物中，GSH储备没有变化。提示高膳食蛋白质和氨基酸对F致敏的保护作用是通过组织GSH升高介导的，尽管Met和Gly的最佳消耗量不同。肝脏FGH活性与RSSL一起可被视为实验性F致敏的敏感生化指标。
