Weber T E, Kerr B J, Spurlock M E
USDA-ARS, National Swine Research and Information Center, Ames, IA 50011-3310, USA.
J Anim Physiol Anim Nutr (Berl). 2008 Oct;92(5):569-77. doi: 10.1111/j.1439-0396.2007.00750.x.
Soy protein regulates adiponectin and peroxisome proliferator-activated receptor alpha (PPARalpha) in some species, but the effect of dietary soy protein on adiponectin and PPARalpha in the pig has not been studied. Therefore, the objective of this study was to determine whether soya bean meal reduction or replacement influences serum adiponectin, adiponectin mRNA, serum metabolites and the expression of PPARalpha and other genes involved in lipid deposition. Thirty-three pigs (11 pigs per treatment) were subjected to one of three dietary treatments: (i) reduced crude protein (CP) diet containing soya bean meal (RCP-Soy), (ii) high CP diet containing soya bean meal (HCP-Soy) or (iii) high CP diet with corn gluten meal replacing soya bean meal (HCP-CGM) for 35 days. Dietary treatment had no effect on overall growth performance, feed intake or measures of body composition. There was no effect of dietary treatment on serum adiponectin or leptin. Dietary treatment did not affect the abundance of the mRNAs for adiponectin, PPARalpha, PPARgamma2, lipoprotein lipase or fatty acid synthase in adipose tissue. The mRNA expression of PPARalpha, PPARgamma2, lipoprotein lipase or fatty acid synthetase in loin muscle was not affected by dietary treatment. In liver tissue, the relative abundance of PPARalpha mRNA was greater (p < 0.05) in pigs fed the HCP-Soy diets when compared to pigs fed RCP-Soy or HCP-CGM diets. Hepatic mRNA expression of acyl-CoA oxidase or fatty acid synthase was not affected by dietary treatment. Western blot analysis indicated that hepatic PPARalpha protein levels were decreased (p < 0.05) in pigs fed the RCP-Soy diets when compared to pigs fed the HCP-Soy diets. These data suggest that increasing the soy protein content of swine diets increases hepatic expression of PPARalpha without associated changes in body composition.
大豆蛋白在某些物种中可调节脂联素和过氧化物酶体增殖物激活受体α（PPARα），但日粮大豆蛋白对猪脂联素和PPARα的影响尚未得到研究。因此，本研究的目的是确定减少或替代豆粕是否会影响血清脂联素、脂联素mRNA、血清代谢产物以及PPARα和其他参与脂质沉积的基因的表达。33头猪（每种处理11头猪）接受三种日粮处理之一：（i）含豆粕的粗蛋白（CP）降低日粮（RCP - 大豆），（ii）含豆粕的高CP日粮（HCP - 大豆）或（iii）用玉米蛋白粉替代豆粕的高CP日粮（HCP - CGM），持续35天。日粮处理对总体生长性能、采食量或身体组成指标没有影响。日粮处理对血清脂联素或瘦素没有影响。日粮处理不影响脂肪组织中脂联素、PPARα、PPARγ2、脂蛋白脂肪酶或脂肪酸合酶的mRNA丰度。日粮处理对腰大肌中PPARα、PPARγ2、脂蛋白脂肪酶或脂肪酸合酶的mRNA表达没有影响。在肝脏组织中，与饲喂RCP - 大豆或HCP - CGM日粮的猪相比，饲喂HCP - 大豆日粮的猪中PPARα mRNA的相对丰度更高（p < 0.05）。日粮处理对酰基辅酶A氧化酶或脂肪酸合酶的肝脏mRNA表达没有影响。蛋白质免疫印迹分析表明，与饲喂HCP - 大豆日粮的猪相比，饲喂RCP - 大豆日粮的猪肝脏中PPARα蛋白水平降低（p < 0.05）。这些数据表明，增加猪日粮中的大豆蛋白含量可增加肝脏中PPARα的表达，而身体组成无相关变化。
