Wasi S, Murray S A, Gill P
National Reference Laboratory, Canadian Red Cross Society, Ottawa.
Vox Sang. 1994;67(1):1-7. doi: 10.1111/j.1423-0410.1994.tb05028.x.
A national proficiency testing program has been established to monitor the accuracy and precision of factor VIII:C (FVIII:C) assays performed at the various Blood Services Centres of the Canadian Red Cross Society involved in the collection and processing of either donor blood designated for component production or plasma destined for fractionation. This paper describes the preparation, design, and results of the first four exercises involving 19 laboratories. The exercises were designed to allow the investigation of intralaboratory variability between replicate samples and precision of assays as well as the causes of interlaboratory variability. The implementation of this program has led to improved precision and interlaboratory agreement on FVIII:C assays. These improvements were achieved mainly as a result of a modification in the method for sample dilution, an increase in both replicate testing of the same sample and the number of sample dilutions, and a reduction in the number of reagent systems used by various centres.
已设立一项全国性能力验证计划，以监测加拿大红十字会各血液服务中心所进行的凝血因子VIII：C（FVIII：C）检测的准确性和精密度。这些中心参与采集和处理用于制备成分血的献血者血液或用于分馏的血浆。本文描述了涉及19个实验室的前四次检测的准备、设计和结果。这些检测旨在研究重复样本间的实验室内变异性和检测精密度，以及实验室间变异性的原因。该计划的实施提高了FVIII：C检测的精密度和实验室间的一致性。这些改进主要是由于样本稀释方法的改变、同一样本重复检测次数和样本稀释次数的增加，以及各中心使用的试剂系统数量的减少。
