Joustra-Dijkhuis A M, Boomgaard M N, Pietersz R N, Steneker I, de Korte D, Loos J A, Reesink H W
Red Cross Blood Bank Amsterdam, The Netherlands.
Vox Sang. 1994;67(1):22-7. doi: 10.1111/j.1423-0410.1994.tb05032.x.
The effect of filtration on the quality of platelet concentrates (PC) during storage was investigated. Two leukocyte depletion filters (Pall PL50HF and Sepacell PL-10A) were applied to filter PC made from a pool of 4 buffy coats. For each experiment 3 PC were pooled and divided into 3 identical PC to eliminate differences between the PC. Two PC were filtered, and the third PC served as an unfiltered control. A total of 12 experiments was performed. Before filtration, volumes of the PC were 263 +/- 11.7 ml (mean +/- SD). Platelet and leukocyte counts per PC were 241 +/- 25.9 x 10(9) and 7.2 +/- 1.8 x 10(6), respectively. After filtration leukocyte counts did not exceed 5 x 10(4) in any of the PC. In the PC filtered with the Pall PL50HF the mean platelet loss was approximately 14% and with the Sepacell PL-10A, 17%. During a 9-day storage period the pH, PO2, PCO2, bicarbonate, lactate and glucose concentration and LDH release as well as the morphology, examined by the swirling effect and microscopically, were not significantly different in filtered and unfiltered units. Filtration through the 2 investigated leukocyte depletion filters for PC did not adversely affect in vitro viability of the platelets during storage.
研究了过滤对血小板浓缩物（PC）储存期间质量的影响。使用两种白细胞去除过滤器（颇尔PL50HF和日水PL-10A）对由4份 Buffy 层制备的PC进行过滤。每次实验将3份PC合并，并分成3份相同的PC以消除PC之间的差异。两份PC进行过滤，第三份PC作为未过滤的对照。共进行了12次实验。过滤前，PC的体积为263±11.7 ml（平均值±标准差）。每份PC中的血小板和白细胞计数分别为241±25.9×10⁹和7.2±1.8×10⁶。过滤后，任何一份PC中的白细胞计数均未超过5×10⁴。在用颇尔PL50HF过滤的PC中，平均血小板损失约为14%，而用日水PL-10A过滤的PC中，平均血小板损失为17%。在9天的储存期内，通过漩涡效应和显微镜检查的过滤和未过滤单位的pH、PO₂、PCO₂、碳酸氢盐、乳酸和葡萄糖浓度以及LDH释放以及形态均无显著差异。通过这两种研究的白细胞去除过滤器对PC进行过滤，在储存期间不会对血小板的体外活力产生不利影响。
