Cherwonogrodzky J W, Knodel M H, Spence M R
Defence Research Establishment Suffield, Albert, Canada.
Vaccine. 1994 Jul;12(9):773-5. doi: 10.1016/0264-410x(94)90284-4.
Francisella tularensis live vaccine strain (LVS), taken directly from lyophilized vials, did not appear to have a capsule and had low virulence for Balb/c mice. When this strain was subcultured on Chamberlain's synthetic medium (pH 6.5), it became extensively capsulated (1-5 micron in diameter) and its virulence for Balb/c mice increased about 1000-fold. We conclude that the virulence of the attenuated vaccine strain, F. tularensis LVS, may still be influenced by growth conditions.
直接从冻干瓶中取出的土拉弗朗西斯菌活疫苗株（LVS）似乎没有荚膜，对Balb/c小鼠的毒力较低。当该菌株在张伯伦合成培养基（pH 6.5）上进行传代培养时，它形成了大量的荚膜（直径为1-5微米），并且其对Balb/c小鼠的毒力增加了约1000倍。我们得出结论，减毒疫苗株土拉弗朗西斯菌LVS的毒力仍可能受生长条件的影响。
