Anna-Arriola S S, Herskowitz I
Graduate Group in Genetics, University of California, Berkeley 94720.
Yeast. 1994 Jun;10(6):801-10. doi: 10.1002/yea.320100610.
We have isolated a mutant which exhibits partial constitutivity for a-specific gene expression in alpha cells. The wild-type gene was cloned and demonstrated to be allelic to the STE13 gene, which encodes the dipeptidyl aminopeptidase involved in processing of the alpha-factor prepropheromone. Thus, the mating defect of the ste13 mutations in alpha cells may result both from the production of incompletely processed alpha-factor and from the increased expression of a-specific genes. The STE13 open reading frame of 931 amino acids contains a putative membrane-spanning segment near its amino terminus and is 31% identical to a second yeast dipeptidyl aminopeptidase (DAP2). A null mutant of STE13 has been constructed: it is viable and sporulation-proficient.
我们分离出了一个突变体，该突变体在α细胞中对a特异性基因表达表现出部分组成型。野生型基因被克隆出来，并证明与STE13基因等位，STE13基因编码参与α因子前体加工的二肽基氨肽酶。因此，α细胞中ste13突变的交配缺陷可能是由于不完全加工的α因子的产生以及a特异性基因表达的增加。STE13的开放阅读框有931个氨基酸，在其氨基末端附近包含一个假定的跨膜区段，与第二种酵母二肽基氨肽酶（DAP2）有31%的同源性。已经构建了STE13的缺失突变体：它是可存活的且产孢能力正常。
