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固定化酶在食品分析中的应用

Immobilized enzymes as tools in food analysis.

作者信息

Schwedt G, Stein K

机构信息

Institut für Anorganische und Analytische Chemie, TU Clausthal, Clausthal-Zellerfeld, Germany.

出版信息

Z Lebensm Unters Forsch. 1994 Sep;199(3):171-82. doi: 10.1007/BF01193438.

Abstract

A lot of publications described the possibilities of using selective enzymatic reactions in analysis, but not much authors described applications for the analysis of real samples. In this paper important publications, which described different applications in food analysis, are reviewed. In the first section the use of biosensors for food analysis, in the second section the combination of immobilized enzymes and flow injection analysis and in the last section the use of immobilized enzymes in combination with HPLC are described. Most of the applications described used enzymes for the determination of sugars mainly glucose, but also methods for the determination of inhibitors in foods are described.

摘要

许多出版物描述了在分析中使用选择性酶促反应的可能性,但没有太多作者描述其在实际样品分析中的应用。本文综述了描述在食品分析中不同应用的重要出版物。第一节介绍了生物传感器在食品分析中的应用,第二节介绍了固定化酶与流动注射分析的结合,最后一节介绍了固定化酶与高效液相色谱联用的应用。所描述的大多数应用使用酶来测定糖类,主要是葡萄糖,但也描述了食品中抑制剂的测定方法。

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