Yang X, Li S, Wang Y
Biology Department of Beijing Normal University.
Shi Yan Sheng Wu Xue Bao. 1994 Jun;27(2):175-81.
Protein B23 is one of the two major protein components in eukaryocytic nucleolus. Previous studies indicate that the cellular content and distribution of protein B23 are correlated with the synthetic rate of ribosomal RNA (rRNA) and cell growth. It is more abundant in tumor and growing cells than in normal resting cells. It may translocate from nucleolus to nucleoplasm after serum deprivation, or after being treated with RNA inhibitors such as actinomycin D, toyocamycin, or doxorubicin. In order to search for the role of protein B23 in cell division, using an anti-B23 monoclonal antibody, we detect the cellular content and distribution of protein B23 in interphase and mitotic HeLa cells, respectively. Two microtubule drugs, colcemid and taxol, are used to arrest the mitotic cells. The results show the cellular content of protein B23 in mitotic cells is much higher than that in interphase cells. The intracellular distribution of protein B23 in mitotic cells is distinguished from that in interphase cells too.
蛋白质B23是真核细胞核仁中两种主要蛋白质成分之一。先前的研究表明，蛋白质B23的细胞含量和分布与核糖体RNA（rRNA）的合成速率及细胞生长相关。它在肿瘤细胞和生长中的细胞中比在正常静止细胞中更为丰富。在血清剥夺后，或在用放线菌素D、丰加霉素或阿霉素等RNA抑制剂处理后，它可能从核仁转移至核质。为了探究蛋白质B23在细胞分裂中的作用，我们使用抗B23单克隆抗体，分别检测了间期和有丝分裂期HeLa细胞中蛋白质B23的细胞含量和分布。使用两种微管药物秋水仙酰胺和紫杉醇来使有丝分裂细胞停滞。结果显示，有丝分裂细胞中蛋白质B23的细胞含量远高于间期细胞。有丝分裂细胞中蛋白质B23的细胞内分布也与间期细胞不同。
