针对促甲状腺激素受体的单克隆抗体的免疫组织化学特性分析

Immunohistochemical characterization of monoclonal antibodies directed against the TSH receptor.

作者信息

Kohnert K D, Krabbe S, Meng W

机构信息

Department of Experimental Endocrinology Institute of Diabetes Gerhardt Katsch University of Greifswald, Karlsburg, Germany.

出版信息

Acta Histochem. 1994 Jun;96(2):175-80. doi: 10.1016/S0065-1281(11)80174-1.

DOI:10.1016/S0065-1281(11)80174-1

PMID:7976127

Abstract

Monoclonal antibodies have been obtained by fusing mouse myeloma cells with spleen cells of mice immunized with crude thyroid membranes. Among the antibodies reactive with different thyroid antigenic components, three were found to specifically react with TSH receptor molecules. These antibodies displayed characteristic staining patterns on frozen sections of thyroid tissue from patients with various thyroid diseases upon identification of antibody binding by indirect peroxidase staining. No specific reactivity was detected with tissue from other human organs, such as pancreas, liver, fat, and muscle. The results demonstrate that the immunoperoxidase technique and the specificity of the monoclonal antibodies produced permitted the identification of cellular constituents that might be important antigens in autoimmune thyroid disease.

摘要

通过将小鼠骨髓瘤细胞与用粗制甲状腺膜免疫的小鼠脾细胞融合，获得了单克隆抗体。在与不同甲状腺抗原成分反应的抗体中，发现有三种与促甲状腺激素（TSH）受体分子特异性反应。通过间接过氧化物酶染色鉴定抗体结合后，这些抗体在患有各种甲状腺疾病患者的甲状腺组织冰冻切片上呈现出特征性染色模式。在来自其他人体器官（如胰腺、肝脏、脂肪和肌肉）的组织中未检测到特异性反应。结果表明，免疫过氧化物酶技术和所产生单克隆抗体的特异性使得能够鉴定出可能是自身免疫性甲状腺疾病中重要抗原的细胞成分。