Sabater J F, Zaragoza J M
Multidisciplinary Analytic Laboratory, Valencia, Spain.
Acta Microbiol Hung. 1993;40(4):343-9.
Based on a twenty-year experience, a simple identification system for slowly growing mycobacteria has been presented for clinical laboratories not specialized in this work. With this system (12 tests: tolerance to 0.02% picric acid, colony pigmentation in the dark, nitrate reduction, resistance to ethambutol, tween hydrolysis at 7 and 14 days, resistance to hydroxylamine, PAS degradation, tolerance to p-nitrobenzoic acid, production of nicotinic acid and colony morphology) we have identified 15 strains of Mycobacterium gordonae and 10 of Mycobacterium avium-intracellulare complex, isolated from surface water in Valencia, Spain.
基于二十年的经验，已为不专门从事这项工作的临床实验室提出了一种用于缓慢生长分枝杆菌的简单鉴定系统。利用该系统（12项检测：对0.02%苦味酸的耐受性、暗处菌落色素沉着、硝酸盐还原、对乙胺丁醇的抗性、7天和14天的吐温水解、对羟胺的抗性、PAS降解、对对硝基苯甲酸的耐受性、烟酸产生及菌落形态），我们鉴定了从西班牙巴伦西亚地表水分离出的15株戈登分枝杆菌和10株鸟分枝杆菌-胞内分枝杆菌复合体。
