Nagai S
Gastroenterol Jpn. 1976;11(4):363-73. doi: 10.1007/BF02777378.
Paraformaldehyde (PF) treatment of epithelial cells of human stomach revealed intense yellow fluorescent granules within the cells. These granules were identified as serotonin on microspectrofluorometry. Serotonin containing cells were also identified as enterochromaffin cells (EC cells) under electron microscope and by the histochemical analysis. O-phthalaldehyde treatment (OPT) of epithelial cells in human stomach demonstrated whitish blue intracellular granules under fluorescence microscope. The cells containing these granules were identified as histamine containing cells on microspectrofluorometry, because of accordance in excitation and emission spectra with authentic histamine. Histamine-containing cells were considered to be identical to the mast cells, because of metachromatic property on toluidine blue staining and of close structural similarities under electron microscope.
用多聚甲醛（PF）处理人胃上皮细胞后，细胞内可见强烈的黄色荧光颗粒。通过显微分光荧光测定法，这些颗粒被鉴定为5-羟色胺。在电子显微镜下以及通过组织化学分析，含5-羟色胺的细胞也被鉴定为肠嗜铬细胞（EC细胞）。用人胃上皮细胞进行邻苯二甲醛处理（OPT）后，在荧光显微镜下可见淡蓝色的细胞内颗粒。通过显微分光荧光测定法，含有这些颗粒的细胞被鉴定为含组胺细胞，因为其激发光谱和发射光谱与纯组胺一致。含组胺细胞被认为与肥大细胞相同，这是基于其对甲苯胺蓝染色的异染特性以及在电子显微镜下结构高度相似。
