用于电子显微镜检查的重金属组织切片染色。II. 含铅和钡溶液的应用。
Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium.
作者信息
WATSON M L
出版信息
J Biophys Biochem Cytol. 1958 Nov 25;4(6):727-30. doi: 10.1083/jcb.4.6.727.
Abstract
Descriptions of three heavy metal stains and methods of application to tissue sections for electron microscopy are presented. Lead hydroxide stains rather selectively two types of particles in liver: those associated with the endoplasmic reticulum and containing ribonucleic acid and other somewhat larger particles. Barium hydroxide emphasizes certain bodies within vesicles of the Golgi region of hepatic cells. Alkalized lead acetate is useful as a general stain, as are also lead and barium hydroxides.
摘要
本文介绍了三种重金属染色剂及其应用于电子显微镜组织切片的方法。氢氧化铅能相当有选择性地对肝脏中的两种颗粒进行染色：一种是与内质网相关且含有核糖核酸的颗粒，另一种是稍大一些的颗粒。氢氧化钡能突出肝细胞高尔基体区域小泡内的某些小体。碱式醋酸铅与氢氧化铅和氢氧化钡一样，可用作通用染色剂。
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本文引用的文献
1
Staining of tissue sections for electron microscopy with heavy metals.用重金属对组织切片进行电子显微镜染色。
J Biophys Biochem Cytol. 1958 Jul 25;4(4):475-8. doi: 10.1083/jcb.4.4.475.
2
Thin sections. II. A simple method for reducing compression artifacts.薄片。II. 一种减少压缩伪影的简单方法。
J Biophys Biochem Cytol. 1958 May 25;4(3):345-8. doi: 10.1083/jcb.4.3.345.
3
Electron microscope study of a cell-free induced leukemia of the mouse: a preliminary report.小鼠无细胞诱导白血病的电子显微镜研究：初步报告。
J Biophys Biochem Cytol. 1958 Mar 25;4(2):151-6. doi: 10.1083/jcb.4.2.151.
4
Reduction of heating artifacts in thin sections examined in the electron microscope.减少在电子显微镜下检查的薄片中的加热伪像。
J Biophys Biochem Cytol. 1957 Nov 25;3(6):1017-22. doi: 10.1083/jcb.3.6.1017.
5
Microbodies and the problem of mitochondrial regeneration in liver cells.微体与肝细胞中线粒体再生的问题。
J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):355-60. doi: 10.1083/jcb.2.4.355.
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