柱切换高效液相色谱法测定炔诺酮-α,β-聚(3-羟丙基)-DL-天冬酰胺缀合物的体内释放量
[Column switching HPLC method for determination of in vivo release of norethindrone-alpha, beta-poly (3-hydroxypropyl)-DL-asparamide conjugate].
作者信息
Tang G P, Chen Q Q
机构信息
Department of Pharmacy, Zhejaing Medical University, Hangzhou.
出版信息
Yao Xue Xue Bao. 1994;29(4):301-5.
An HPLC method for the determination of in vivo release of norethindrone-alpha, beta-poly (3-hydroxypropyl)-DL-asparamide (NET-PHPA) conjugate was established using column switching technique. The sample prepurification could be omitted. It was extracted by a precolumn (ODS, 9-11 microns) and the analytical column was packed with ODS (15 cm x 4 mm ID. Shimpac CLC). The mobile phases were water and methanol--water (7:3) respectively. The experiment showed that the recovery from serum was 82.6%, the RSD within day was 1.3-1.8% and that in day-to-day was 0.7-5.6% (n = 4).
采用柱切换技术建立了一种测定炔诺酮-α，β-聚（3-羟丙基）-DL-天冬酰胺（NET-PHPA）缀合物体内释放的高效液相色谱法。可省略样品预纯化步骤。样品通过预柱（ODS，9 - 11微米）萃取，分析柱填充ODS（15厘米×4毫米内径，Shimpac CLC）。流动相分别为水和甲醇 - 水（7:3）。实验表明，血清回收率为82.6%，日内相对标准偏差为1.3 - 1.8%，日间相对标准偏差为0.7 - 5.6%（n = 4）。
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