大鼠正中隆起提取物对大鼠体内生长激素分泌的影响。

Effect of rat stalk median eminence extracts on rat GH secretion in vivo.

作者信息

Collu R, Taché Y, Du Ruisseau P, Ducharme J R

出版信息

Horm Res. 1976;7(4-5):274-9. doi: 10.1159/000178738.

Abstract

Estradiol (0.2 mug), injected subcutaneously for 10 days to adult male rats, increased plasma growth hormone (GH) levels as compared with oil-treated controls. In estradiol-pretreated (10 days), urethane-anesthetized rats, the first as well as the second of two successive intracarotid injections, at 1-hour intervals, of one rat stalk median eminence equivalent evoked a significant rise in plasma radioimmunoassayable GH. Under the same conditions, cerebral cortex extracts (1 equivalent) induced a slight elevation whereas vasopressin (30 mU) or serotonin (200 ng) were ineffective. These results indicate that estrogen-primed, urethane-anesthetized rats can be used to demonstrate GH-releasing activity in rat SME extracts.

摘要

对成年雄性大鼠皮下注射雌二醇（0.2微克），连续注射10天，与注射油的对照组相比，血浆生长激素（GH）水平升高。在预先用雌二醇处理10天的、氨基甲酸乙酯麻醉的大鼠中，每隔1小时进行两次连续颈内注射，每次注射相当于一个大鼠正中隆起的提取物，第一次和第二次注射均引起血浆中可通过放射免疫测定的GH显著升高。在相同条件下，大脑皮质提取物（1个当量）引起轻微升高，而血管加压素（30 mU）或5-羟色胺（200 ng）则无效。这些结果表明，雌激素预处理、氨基甲酸乙酯麻醉的大鼠可用于证明大鼠正中隆起提取物中的生长激素释放活性。