Clemo F A, DeNicola D B, Zimmermann J L
Department of Pathobiology, School of Veterinary Medicine, Purdue University, West Lafayette, IN 47907.
Am J Vet Res. 1994 Aug;55(8):1139-47.
Monoclonal antibody (MAB) B72.3, which recognizes human tumor-associated glycoprotein-72, has immunoreactivity for malignant epithelial neoplasms in human beings and dogs. To further characterize the range of immunoreactivity of MAB B72.3 in canine tissues, MAB B72.3 and 2 other tumor-associated glycoprotein-72 antibodies (MAB CC49 and CC83) were tested against a wide spectrum of normal tissues from dogs. Immunoreactivity was detected, using an avidin-biotin-complex immunoperoxidase method. Monoclonal antibody B72.3 did not stain most types of normal canine tissues, but various types of epithelial cells within the gastrointestinal and respiratory tract mucosae, salivary gland, esophagus, epididymis, uterus, thymus, hair follicle, and apocrine glands of the anal sac had variable staining with MAB B72.3. A similar range of immunoreactivity in comparable types of normal tissues was seen for MAB CC49 and CC83; however, MAB CC49, but not MAB B72.3 and CC83, stained the endothelium of capillaries and small vessels in most normal tissues. Staining of frozen and paraffin-embedded tissues was similar. In conclusion, we found that MAB B72.3, CC49, and CC83 had selected immunoreactivity for specific types of normal canine epithelial cells, especially those involved with mucin production.
识别人类肿瘤相关糖蛋白-72的单克隆抗体（MAB）B72.3，对人类和犬类的恶性上皮肿瘤具有免疫反应性。为了进一步明确MAB B72.3在犬类组织中的免疫反应范围，使用MAB B72.3和其他2种肿瘤相关糖蛋白-72抗体（MAB CC49和CC83）对犬类的多种正常组织进行检测。采用抗生物素蛋白-生物素复合物免疫过氧化物酶法检测免疫反应性。单克隆抗体B72.3对大多数类型的正常犬类组织无染色，但胃肠道和呼吸道黏膜、唾液腺、食管、附睾、子宫、胸腺、毛囊和肛门囊顶泌腺内的各种上皮细胞对MAB B72.3有不同程度的染色。MAB CC49和CC83在类似类型的正常组织中也有类似的免疫反应范围；然而，MAB CC49可使大多数正常组织中的毛细血管和小血管内皮染色，而MAB B72.3和CC83则不能。冷冻组织和石蜡包埋组织的染色情况相似。总之，我们发现MAB B72.3、CC49和CC83对特定类型的正常犬类上皮细胞，尤其是那些与粘蛋白产生有关的细胞具有选择性免疫反应性。
