肿瘤蛋白MAGE-1位于人黑素瘤细胞的胞质溶胶中。
The tumor protein MAGE-1 is located in the cytosol of human melanoma cells.
作者信息
Amar-Costesec A, Godelaine D, Stockert E, van der Bruggen P, Beaufay H, Chen Y T
机构信息
International Institute of Cellular and Molecular Pathology, Université de Louvain, Brussels, Belgium.
出版信息
Biochem Biophys Res Commun. 1994 Oct 28;204(2):710-5. doi: 10.1006/bbrc.1994.2517.
The MAGE-1 protein has been localized to the cytosol of human melanoma culture cells (MZ2-MEL.43) by subcellular fractionation. Its distribution between nuclear, mitochondrial, microsomal and cytosolic fractions was established by SDS-PAGE and immunoblotting with a rabbit antiserum and compared to that of markers for the main cell compartments. The immunoreactive material was recovered in the cytosolic fraction as a polypeptide migrating at 42 kDa, which has been further identified as the MAGE-1 protein because it comigrates with the product of cell-free transcription-translation of the MAGE-1 cDNA. Location to the cytosol was confirmed by immunofluorescence microscopy.
通过亚细胞分级分离法，MAGE-1蛋白已定位到人类黑色素瘤培养细胞（MZ2-MEL.43）的细胞质中。通过SDS-PAGE以及用兔抗血清进行免疫印迹，确定了其在细胞核、线粒体、微粒体和细胞质组分之间的分布，并与主要细胞区室的标志物分布进行了比较。免疫反应性物质在细胞质组分中作为一条迁移率为42 kDa的多肽被回收，该多肽已被进一步鉴定为MAGE-1蛋白，因为它与MAGE-1 cDNA的无细胞转录-翻译产物迁移率相同。通过免疫荧光显微镜证实了其在细胞质中的定位。
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