Sawada H, Tsusué M, Iino T
Biological Laboratory, School of Liberal Arts and Science, Kitasato University, Japan.
Biol Chem Hoppe Seyler. 1994 Jun;375(6):425-7. doi: 10.1515/bchm3.1994.375.6.425.
An ommin was isolated from the integument of the quail mutant of the silkworm, Bombyx mori, using column chromatography on SP-Sephadex and cellulose powder. As a standard, ommin was isolated from diapause eggs of the normal silkworm. The red pigment from the larval integument of quail mutants was identical to the standard compound with respect to absorption spectrum, infrared spectrum and RF values in thin-layer chromatography (TLC). After acid hydrolysis of the pigment, 3-hydroxykynurenine was detected by TLC. This is the first report of an ommin in a lepidopteran larval integument.
利用SP-葡聚糖凝胶和纤维素粉进行柱色谱法,从家蚕鹌鹑突变体的体表分离出了一种眼色素。作为标准品,眼色素是从正常家蚕的滞育卵中分离出来的。鹌鹑突变体幼虫体表的红色素在吸收光谱、红外光谱和薄层色谱(TLC)中的比移值方面与标准化合物相同。对该色素进行酸水解后,通过TLC检测到了3-羟基犬尿氨酸。这是首次在鳞翅目幼虫体表报道眼色素。