Shaha C
Sperm Biotechnology Laboratory, National Institute of Immunology, New Delhi, India.
Mol Reprod Dev. 1994 Aug;38(4):393-403. doi: 10.1002/mrd.1080380407.
Interaction of specific ligands with cell surface molecules may induce reorganization of surface components. A monoclonal antibody (B-12) against sperm surface antigens of 40kDa size induced molecules on the plasma membrane overlying the acrosome of rabbit sperm to cluster in small aggregates at 0 degree C (patching). At an elevated temperature of 37 degrees C these clusters of antigen antibody complexes collected into a large aggregate on one pole of the cell forming a cap (capping). This cap-like structure showed a reduction in size over a period of time and eventually disappeared from the sperm surface. Inhibition of capping by sodium azide indicated that it is an energy-dependent process. Patching of antigens did not require energy. Involvement of sperm head cytoskeleton in the process of capping was evident from potentiation of cap formation by cytoskeleton disrupting agents like cytochalasin B and D. Patching of antigen antibody complexes was not affected by either of the agents. The loss of antigen antibody complexes from sperm surface was mainly due to shedding of the complexes in the surrounding media. Sperm with patches of antigen antibody complexes did not adhere to oocytes. Sperm from the group where a majority of the sperm were denuded of the antigen antibody complexes also did not bind to oocytes.
特定配体与细胞表面分子的相互作用可能会诱导表面成分的重组。一种针对40kDa大小精子表面抗原的单克隆抗体（B - 12），在0℃时可诱导覆盖兔精子顶体的质膜上的分子聚集成小聚集体（贴片）。在37℃的升高温度下，这些抗原 - 抗体复合物簇聚集在细胞一极形成一个大聚集体，形成帽状结构（盖帽）。这种帽状结构在一段时间内尺寸减小，最终从精子表面消失。叠氮化钠对盖帽的抑制表明这是一个能量依赖的过程。抗原的贴片不需要能量。细胞松弛素B和D等细胞骨架破坏剂增强帽的形成，这表明精子头部细胞骨架参与了盖帽过程。抗原 - 抗体复合物的贴片不受这两种试剂中任何一种的影响。精子表面抗原 - 抗体复合物的丢失主要是由于复合物脱落到周围介质中。带有抗原 - 抗体复合物贴片的精子不与卵母细胞粘附。大多数精子去除了抗原 - 抗体复合物的组中的精子也不与卵母细胞结合。
