Càceres C, Ribes E, Muller S, Cornudella L, Chiva M
Department Enginyeria Química, Universitat Politècnica de Catalunya, Barcelona, Spain.
Mol Reprod Dev. 1994 Aug;38(4):440-52. doi: 10.1002/mrd.1080380412.
During the process of chromatin condensation in the spermiogenesis of the neogastropod mollusc Murex brandaris, the nuclear protein complement undergoes a complex series of changes. These changes lead to the appearance of three small protamines in the ripe sperm nuclei. We have characterized this system electrophoretically and at the compositional level, as well as through the analysis of crossreactions with antibodies elicited against a specific spermatozoan protamine. Our results indicate that the complex pattern of chromatin condensation during spermiogenesis in this species (M. brandaris) may be modulated by a series of post-translational (and intranuclear) modifications of DNA-interacting proteins, such as precursors to the sperm protamines. The amino acid composition of each sperm protamine is remarkably simple (lys + arg + gly > or = 96 mol%). This system of spermiogenic/spermatozoal proteins in the neogastropod M. brandaris clearly differs from that in patellogastropods and archaeogastropods, and it may be helpful in understanding evolutionary changes in the chromatin condensation pattern during the spermiogenesis of gastropod molluscs.
在新腹足类软体动物斑纹骨螺精子发生过程中的染色质浓缩过程中，核蛋白成分经历了一系列复杂的变化。这些变化导致成熟精子细胞核中出现三种小的鱼精蛋白。我们通过电泳、成分分析以及与针对特定精子鱼精蛋白产生的抗体的交叉反应分析，对该系统进行了表征。我们的结果表明，该物种（斑纹骨螺）精子发生过程中染色质浓缩的复杂模式可能受到与DNA相互作用蛋白（如精子鱼精蛋白前体）的一系列翻译后（和核内）修饰的调节。每种精子鱼精蛋白的氨基酸组成非常简单（赖氨酸+精氨酸+甘氨酸≥96摩尔%）。新腹足类斑纹骨螺的这种精子发生/精子蛋白系统明显不同于笠贝腹足类和古腹足类，它可能有助于理解腹足类软体动物精子发生过程中染色质浓缩模式的进化变化。
